From: Steve Merlin (merlin@aitken.org)
Date: Tue Jul 28 1998 - 09:30:00 EST
Eric, To eliminate moisture in the air line for the Vantage and protect the sample differential regulator, I purchased a Drierite plastic drying column from VWR Scientific, Tel : 1-800-932-5000 Cat. # 26668-007 (Mfg #26800). The dessicant will last several days before needing recharging or replacement. Refill dessicant is the 8 mesh size, Cat# 22891-040 (mfg #23005). The cost is around $40.00 for the drying tube. The other option is to use bottled nitrogen as the pressure source. I found the drierite method to be sufficient to solve the problem and easy to maintain. Steven Merlin Aitken Neuroscience Center Flow Cytometry Facility 523 East 72nd Street/8th Floor New York, N.Y. 10021 212-772-6426 (Lab) 212-772-0608 (Office) 212-772-0357 (Fax) merlin@aitken.org Eric Van Buren wrote: > >Last Friday we had the sample differential on our FACS Vantage fail. It >appears to have "frozen up" due to some mineral deposits. Has anyone tried >to clean one of these? Is it practical? > >The source of the deposits appears to be our pressure pump. On humid days, >it blows moist air through the cytometer. Has anyone else dealt with this >sort of problem? I'm interested in hearing remedies before I "re-invent the >wheel". > >Thanks, >Eric > >/\/\/\_ Eric Van Buren, aa9080@wayne.edu >\ \ \ Karmanos Cancer Institute and Immunology & Microbiology > \_^_/ Wayne State University, Detroit, Michigan, USA > > > > >RFC822 header >----------------------------------- > >Received: from flowcyt.cyto.purdue.edu (flowcyt.cyto.purdue.edu [128.210.60.31]) > by crane.prod.itd.earthlink.net (8.8.7/8.8.5) with ESMTP id UAA25187 > for <merlin@aitken.org>; Mon, 27 Jul 1998 20:03:18 -0700 (PDT) >Received: (from root@localhost) > by flowcyt.cyto.purdue.edu (8.9.1/8.8.7) id SAA05541 > for cyto-sendout; Mon, 27 Jul 1998 18:50:13 -0500 >Received: (from bin@localhost) > by flowcyt.cyto.purdue.edu (8.9.1/8.8.7) id KAA03505 > for kelley; Mon, 27 Jul 1998 10:55:08 -0500 >Received: from mail4.wayne.edu (mail4.wayne.edu [141.217.1.82]) > by flowcyt.cyto.purdue.edu (8.9.1/8.8.7) with ESMTP id KAA03501 > for <cytometry@flowcyt.cyto.purdue.edu>; Mon, 27 Jul 1998 10:55:07 -0500 >Received: from [146.9.72.237] ([146.9.72.237]) > by mail4.wayne.edu (8.8.5/8.8.5) with SMTP id LAA10980 > for <cytometry@flowcyt.cyto.purdue.edu>; Mon, 27 Jul 1998 11:54:03 -0400 (EDT) >Message-Id: <v01540b01b1e25e7777f0@[146.9.72.237]> >Mime-Version: 1.0 >Content-Type: text/plain; charset="us-ascii" >Date: Mon, 27 Jul 1998 11:53:45 -0500 >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >From: aa9080@wayne.edu (Eric Van Buren) >Subject: Sample Differential Failure >X-UIDL: aa398ce790b747fefa974c2920c5ed16 >
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