From: Mario Roederer (Roederer@beadle.stanford.edu)
Date: Mon Jul 27 1998 - 23:03:03 EST
Dorte: There is no need to use the same sample type for compensation controls as for your experiment. Thus, you can stain a lysed sample (or even murine lymphocytes, if you desire!) with appropriate compensation standards, set the compensation, and then run your unlysed sample. Because the compensation setting depends only on the fluorescence spectrum of the dyes you are using, proper compensation is set whenever you compensate correctly on ANY sample that has been stained solely with that dye. This is true no matter what type of sample you are using, and no matter what the autofluorescences of the various cell types are. (As as your compensation sample has positive and negative or dim cells that have the same autofluorescence as each other... it doesn't matter what they are compared to the actual samples you are running). mr
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