From: Ray Hicks (rh208@cus.cam.ac.uk)
Date: Tue Jul 28 1998 - 06:58:31 EST
Hi Eric, it might also be worth checking the piping in your pressure tank:
The Vantage in my lab had the same thing happen, I discovered that the air
line into the buffer tank was submerged below the surface of the liquid as
is the buffer outlet pipe (I later discovered a couple of dead regulators
in a drawer so it must have happened before). When the external air
pressure was switched off, some buffer flowed back up the air line into the
diaphragm of the regulator and killed it. I moved the air line to a
different port on the sheath tank, (which doesn't have a dipping pipe on
the back of it) and everything's been fine since.
Ray
At 11:53 am -0500 27/7/98, Eric Van Buren wrote:
>Last Friday we had the sample differential on our FACS Vantage fail. It
>appears to have "frozen up" due to some mineral deposits. Has anyone tried
>to clean one of these? Is it practical?
>
>The source of the deposits appears to be our pressure pump. On humid days,
>it blows moist air through the cytometer. Has anyone else dealt with this
>sort of problem? I'm interested in hearing remedies before I "re-invent the
>wheel".
>
>Thanks,
>Eric
>
>/\/\/\_ Eric Van Buren, aa9080@wayne.edu
>\ \ \ Karmanos Cancer Institute and Immunology & Microbiology
> \_^_/ Wayne State University, Detroit, Michigan, USA
Ray Hicks
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