From: Chris Bridts (bridts@uia.ua.ac.be)
Date: Thu Jul 09 1998 - 03:47:28 EST
Dear Avis, Yes, we had the same problem some years ago. We found that sodium heparin tubes are not completely endotoxin free. When we looked at adhesion molecules like CD11B/CD18 they were always upregulated in comparison with EDTA tubes or pharmaceutical heparin. Since then we use EDTA blood for all our functional assays, spinning the buffy coat cells 2x at low speed (200 g) to wash away the platelets. Hopefully this helps chris bridts > Hello: > > I would like to know if anyone has had problems with whole blood clumping in > CPD and Sodium Heparin tubes when they are spun for buffy cells. I have had > unsuccessful attempts with white and platelets aggragation. This problem > does not happen with EDTA blood. > > I am using 7 ml tubes of blood. I spin the tubes at 4000 rpm for 7 minutes. > What could I be doing wrong. > > If anyone could answer this question please respond to my request. > > > > Avis Wooley > MKI Industries > Chris Bridts Laboratory of Immunology University of Antwerpen - UIA Universiteitsplein 1 B2610 Antwerpen Belgium tel.: +32.3.8202594 fax.: +32.3.8202655
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