From: David L. Haviland, Ph.D. (dhavilan@imm2.imm.uth.tmc.edu)
Date: Mon Jul 06 1998 - 11:59:53 EST
Greetings Fellow Cytometrists: I am working with Dr. Dorothy Lewis who chairs the ISAC Data Presentation Standards Committee whose aim is to derive a consensus concerning the presentation of flow cytometry data in the literature. As a result, I have a request I'd like to make from each of you. Through the years, many of us have seen published flow cytometry data that was presented badly, or just outright wrong. These errors could be due, in part, to improper machine "set-up", minimal event collection, or just plain sloppy presentation. We are interested in reviewing any published paper in which you feel errors of any kind reside in the presentation of flow cytometric data. We are looking for any papers you feel would make appropriate (bad) examples. We would be interested in any examples that you have, or find. As a guideline to what we are looking for: 1) Data Interpretation: - Examples where statistics are inappropriately or incorrectly used with either single or two parameter histograms. - Examples where conclusions are drawn with "quantitative" data but are presented without statistical analysis. - Inappropriate, or lack of use markers or regions. 2) Examples where instrument set-up using compensation was not described or incorrectly used after data was obtained. 3) Any example where the conclusion in a paper is in doubt due to improper use of flow cytometry. 4) ANYTHING that you feel is a substandard use, presentation of, or mis-use of flow cytometry. What we'd like to ask is that you send any examples to us at the address below. We would like to obtain as many examples as possible from any and all aspects of flow cytometry - basic science, clinical science, one parameter, two parameter, DNA analysis, or anything. Additionally, I would encourage you to feel free to email the reference to me as well. Feel free to state what you think is wrong with the examples you are submitting. We would like to state for the record that ALL submitted examples WILL be kept CONFIDENTIAL with regard to who sent them. If there are any questions or concerns, please feel free to phone or email me, or Dr. Lewis who is Chair of the ISAC Data Presentation Standards Committee (dlewis@bcm.tmc.edu). Many thanks and we look forward to receiving your submissions. David ============================= David L. Haviland, Ph.D. Asst. Prof. Immunology University of Texas - Houston, H.S.C. Institute of Molecular Medicine 2121 W. Holcombe Blvd. Houston, TX 77030 Internet:"dhavilan@imm2.imm.uth.tmc.edu" Voice: 713.500.2413 FAX: 713.500.2424 ------------------------------------------------------ Never take life seriously. Nobody gets out alive anyway. ============================= -- End --
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