From: T. Vincent Shankey (Thomas Shankey) (TSHANKE@wpo.it.luc.edu)
Date: Mon Jul 06 1998 - 10:43:47 EST
Dear Dr. Lin, In response to your questions regarding platelet function testing in patients undergoing cardiac bypass surgery, a number of studies (flow and non) have looked at platelet and coagulation parameters during bypass. Our own experiance (see Ann Thorac. Surg. 61:1188, 1996) indicates that: 1) aprotinin maintains platelets unstimulated during bypass, and maintains the von-Willebrand pgIb mediated platelet activation pathway; and, 2) analysis of platelet-WBC aggregates are more revealing than platelet-platelet aggregates. Regarding the LIBS issue, most of the antibodies available thus far appear to be sensitive to fixation, making activation related studies a bit difficult (ie, activating the sample with an agonist to determine the platelet "activatability"). For some information regarding LIBS (and RIBS), please see the European Working Group Consensus on Platelet Flow Cytometry (Thromb. Haemost. 79:885, 1998). Vincent Shankey Loyola Univ Med Cntr -- End --
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