From: Ray Hicks (rh208@cus.cam.ac.uk)
Date: Tue Jun 30 1998 - 14:25:54 EST
Hi Marcus,
Be wary of FSC calibration using beads, depending on what they're made of,
they don't necessarily give the same amount of FSC per diameter as cells
would. It would be more realistic to use beads to calibrate time-of-flight
based size measurements (eg FSC-Width or Fluorescence-Width) if your
machine will measure it.
Ray
At 11:32 am +0000 30/6/98, Marcus Reckermann wrote:
>>Has anyone used any of the bead kits to calibrate the forward
>>scatter for size measurements? What was your experience with the
>>kit? Did you use the kit from Flow Cytometry Standards Corp., or
>>from some other company?
>
>Eric,
>I have used Polysciences´ Polybead Polystyrene Beads in various sizes
>>from 0.5 to 15µm, and they each gave multi peak signals, which I
>found very irritating, even after extensive sonicating. However, for
>each size there was a major peak which I then took as the nominal
>size, and that worked out OK. But still, I´m interested in other
>people´s experiences with other products.
>marcus
>__________________________________________________
>Dr. Marcus Reckermann
>Forschungs- und Technologiezentrum Westküste (FTZ)
>Hafentörn
>25761 Büsum
>Tel: 04834-604-204 oder -261
>Fax: 04834-604-299
>E-Mail: recker@ftz-west.uni-kiel.de
Ray Hicks
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