From: Karel Drbal (drbal@leuko.biomed.cas.cz)
Date: Mon Jun 29 1998 - 04:25:08 EST
Try to strip the fluorochrome labelled Ab using ice-cold acid isotonic citrate/phosphate buffer (pH 3.0) (0.263M citric acid/0.123M HPO4 + protein) for a very short time (10 seconds - 1 minute) and neutralize the cell suspension immediately. Spin down the cells, aspirate all supernatant and wash once more and restain with another Ab. This treatment should not harm your sorted cells. Or do you want to strip just the fluorochrome from the bound Ab? Hope this helps. Ken McDonald wrote: > Hi Flowers...I had a question from a Facs user who wants to know if > after sorting a cell population with let's say a FITC stained antibody, > is there a way of then stripping that fluorocrome and re-staining with > another FITC stained antibody, all the while leaving the cell viable? > She needs this to happen right after sorting. > Thankyou in advance...Ken > > -- End -- -- Karel Drbal Laboratory of Leukocyte Antigens Institute of Molecular Genetics Academy of Sciences of the Czech Republic Videnska 1083 142 20 PRAHA 4 Czech Republic, Europe voice: +420-2-4752589 fax: +420-2-44472282 home: +420-2-67315204 e-mail: drbal@leuko.biomed.cas.cz WWW: http://leuko.biomed.cas.cz
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