From: Robert Nordon (m0000771@pop3.unsw.edu.au)
Date: Fri Jun 19 1998 - 00:15:46 EST
Dear Derek, Geoff Osborne has alluded to a common problem when staining non-lymphoid cells: If there is homogeneous staining of the cell type then it is not always possible to "see" equally spaced divisional clusters on CFSE histograms corresponding to consecutive cell divisions (as observed by Bruce Lyons). We have found that it is possible to overcome this problem by sorting CFSE labeled cells the day after staining (set a gate that is <10/256 channels wide). Then you can get crisp divisional peaks with virtually any suspension culture cell type. (See British Journal of Haematology 1997; 98:528-539). Robert Nordon Graduate School of Biomedical Engineering UNSW
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