Re: cell cycle staining - DAPI

From: J.Lawry@sheffield.ac.uk
Date: Fri Jun 19 1998 - 03:22:23 EST

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Dear Sonja

We use DAPI on fixed cells at approx. 2ug/ml adding 20ul per test and
allowing them to stain overnight. Fresh cells need a permeabilization stage
(0.2% Triton X-100 in PBS for example. Add 100ul and mix for one minute)
then add DAPI and run within 20 minutes. Fresh cell staining is very rapid.

Best wishes, John.

 Dr John Lawry,
Institute for Cancer Studies, University Medical School, Beech Hill Road,
Sheffield. S10 2RX.
Tel:0114-271-3023 (lab), 0114-271-3774 (office)
Fax:0114-271-3515
Email: J.Lawry@sheffield.ac.uk
Web page: http://www.shef.ac.uk/uni/academic/A-C/csi/Staff/John/JL.html

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