From: Joanne Yetz-Aldape (jyetz-aldape@genetics.com)
Date: Mon Jun 08 1998 - 12:20:17 EST
Hello, Flowers,
I am having some difficulties with sterility of cells
sorted on my FACStar PLUS. After running 10% bleach and 70% ethanol
through the system, one investigator still reports having bacterial
contamination in the sheath flow-through, plating the sheath in
gentamicin. Another investigator reported that her cells were
overrun with bacteria after 4 days in culture with pen/strep, when
the sorter had been cleaned in the same way, but after one other
cell prep had gone through. The weird thing is that I have been
sorting this one cell prep every week for quite a
while, and there is no contamination in these cultures after 2 weeks
with gentamicin. Could there be a bug I can't remove from the sorter
that is resistant to pen/strep, and is there a critical level of
gentamicin that works in this one situation where the sortied cells
seem clean? Is there some detergent that I could use to purge my
system, or is this something that should just be treated with
antibiotics? I do add gentamicin to my sheath tank, also. Are there
any special sterility problems associated with the turbo sort option??
Any advice or comments would be welcome. Thanks.
Joanne
jyetz-aldape@genetics.com
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