From: Mark A. KuKuruga (kukuru@umich.edu)
Date: Wed Jun 03 1998 - 17:04:01 EST
I've seen suspension grown cells that tend to exist as clumps, yet if you wash them with cold PBS these clumps will often dissociate. Something to try. MAK. Simon_Q_Rice@sbphrd.com wrote: > To: cyto-inbox > cc: cytometry@flowcyt.cyto.purdue.edu > From: Simon Q Rice @ SB_PHARM_RD > Date: 03-Jun-98 08:32:01 AM > Subject: Re: HEK in suspension > Categories: > > Derek, > In my limited experience HEK 293 cells are notoriously difficult ne > impossible to put into suspension without some clumping. I have heard > people say that they can even grow these cells in suspension culture > without clumps, but I remain to be convinced (but if anyone knows > differently I'd like to know please!). > > Could you filter out the large clumps? I recently posted a request to this > effect looking for luer lock filters and have subsequently purchased some > 50um filcons from DAKO in an attempt to overcome clogging, etc. caused by > large clumps of cells. > > Hope this is helpful. > > Simon -- Mark A. KuKuruga, Managing Director University of Michigan Core Flow Cytometry kukuruga@medmail.med.umich.edu
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