From: Michel Canton (mcanton@wcube.fr)
Date: Tue May 19 1998 - 03:05:50 EST
Margaret, I suggest that you read the following reference: F. Dignat-George, et al Rickettsia conorii infection enhances vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 dependent mononuclear cell adherence to endothelial cells The Journal of Infectious Diseases, 1997; 175: 1142-1152 They used our QIFI technology distributed thru DAKO. The main research activity of Professor Jose Sampol is on endothelial cells and quantitative flow cytometry on these cells. You may also contact Dr Philippe Poncelet, Director of Research at BioCytex. He has also a good 10 year experience on this topic. Contact him at: biocytex@biocytex.com Good luck Michel M. Canton, PharmD Managing Director BioCytex >Hi all, We have a FACScan and we want to do quantitative flow on >endothelial cells. The problem is is that the forward scatter settings are >E0 for the beads and E-1 for the cells. Also, the cells are very >autofluorescent so we have to change the voltage for FL1 and FL2 to >accomodate the cells. What is the best way (if any) to use beads to >quantify receptors? Margaret > > >
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