From: Gregor Rothe (gregor.rothe@klinik.uni-regensburg.de)
Date: Thu May 14 1998 - 04:01:41 EST
We recently tried to get hands on Cy7 but could not find any commercial source for it. Amersham indicated that they would no longer distribute Cy7. Also Caltag which distributes PECy7-tandems could not recommend any source for the Cy7 component. Can anyone help with a source for the reagent? Thanks, Gregor Rothe Date: Wed, 13 May 1998 13:46:10 +0000 (PDST) From: BIGOS@Beadle.Stanford.edu Subject: Re: cy7 To: cyto-inbox <cytometry@flowcyt.cyto.purdue.edu> Cc: CYTOMETRY:;;@Beadle.Stanford.edu See Roderer, et al. in Cytometry, V24,#3 for absorbtion and emission spectra of cy7. My reading of Fig. 1 from that paper shows approximately 10% absorbance at 600 nm, so the excitation would be fairly inefficient. The tandem conjugate described, cy7apc, does excite quite well at 600 nm.; it it used regularly here for immunophenotyping directly conjugated to anti -mouse and -human mABs. The emission of cy7 is above 750 nm. This requires a red-sensitive pmt (e.g Hamamatsu 3896) for good detection. -Marty Bigos Stanford Shared FACS Facility ++++++++++++++++++++++++++++++++++++++++++ >>anyone know if cy7 excites with 600nm light and where it emits? >> >>thanks *********************************************************** Institute for Clinical Chemistry and Laboratory Medicine University of Regensburg Franz-Josef-Strauss-Allee 11 D-93053 Regensburg, Germany Tel. +49 (941) 944-6204 Fax +49 (941) 944-6202 E-mail: Gregor.Rothe@KLINIK.UNI-REGENSBURG.DE ***********************************************************
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