From: BIGOS@Darwin.Stanford.EDU
Date: Wed May 13 1998 - 08:46:10 EST
See Roderer, et al. in Cytometry, V24,#3 for absorbtion and emission spectra of cy7. My reading of Fig. 1 from that paper shows approximately 10% absorbance at 600 nm, so the excitation would be fairly inefficient. The tandem conjugate described, cy7apc, does excite quite well at 600 nm.; it it used regularly here for immunophenotyping directly conjugated to anti -mouse and -human mABs. The emission of cy7 is above 750 nm. This requires a red-sensitive pmt (e.g Hamamatsu 3896) for good detection. -Marty Bigos Stanford Shared FACS Facility ++++++++++++++++++++++++++++++++++++++++++ >>anyone know if cy7 excites with 600nm light and where it emits? >> >>thanks
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