quantitative flow

From: Margaret Tropea (mtropea@nih.gov)
Date: Mon May 04 1998 - 15:29:14 EST

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Hi all,  We have a FACScan and we want to do quantitative flow on
endothelial cells.  The problem is is that the forward scatter settings are
E0 for the beads and E-1 for the cells.  Also, the cells are very
autofluorescent so we have to change the voltage for FL1 and FL2 to
accomodate the cells.  What is the best way (if any) to use beads to
quantify receptors?  Margaret

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