Intracellular staining with biotinylated MAbs

From: G R Barclay (Robin_Barclay@compuserve.com)
Date: Thu Apr 23 1998 - 09:56:52 EST

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Has anyone successfully used biotinylated antibodies followed by
streptavidin-fluorochrome conjugates for intracellular staining in fixed
permeabilised leucocytes for flow cytometry?  If so, can you supply
methodology and/or references.
Many thanks
Robin Barclay
SNBTS: Edinburgh

• Next message: William D. Wansley: "3-color leukemia/lymphoma antibody cocktails -Reply"
• Previous message: Theresa L. Fecik: "3-color leukemia/lymphoma antibody cocktails"
• Next in thread: Calman Prussin: "RE: Intracellular staining with biotinylated MAbs"
• Maybe reply: Calman Prussin: "RE: Intracellular staining with biotinylated MAbs"
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