From: Marco Antonio Fernández (mafernandez@mx2.redestb.es)
Date: Sun Apr 19 1998 - 16:36:37 EST
Hi, Nancy I too have been doing PAIg over these years. My experience is that fixed platelets before stain may be stored at 4º C up to 48 hours. False positive or increased fluorescence could arise after this time. Once stained, I recommend you to acquire samples as soon as posible, within 24 hours. Good luck. Marco A. Fernández Biologist Head Flow Cytometry Dept. Reference Laboratory Internet: http://personal.redestb.es/marcoafdez Clinical Cytometry mafernandez@mx2.redestb.es 08024 Barcelona (Spain) ---------- > De: Nancy Barney <nbarney@nih.gov> > A: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > Asunto: > Fecha: sábado 18 de abril de 1998 17:06 > > > Hi Flow folks( anybody doing platelets) : A question from one fairly new to > Flow, we have been doing Platelet Associated Immunoglobulins for a few > months, and the question arose how long can the fixed platelets stay usable > at 4 deg ? In other words once they are fixed ( not stained ) do they have > to be stained the next day ? Also once stained must they be acquired the > same day or is there 24 or 48 hours leeway. Any info appreciated. > > > Nancy Barney MT (ASCP) > medical technologist > 10 2C 390 > 496-6891 > 496-4475 > nbarney@nih.gov >
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