From: Jim Jett (jett@lanl.gov)
Date: Wed Apr 15 1998 - 15:02:04 EST
Claude; In addition to Howard's comments, you might look a what happens to the 'plane' of light scattered by the stream when you are in the sorting mode. If the plane broadens when you turn on the PZT drive, you might be getting too much laser light into the detectors. As Howard suggested, lower the PZT drive amplitude. Also, it might be necessary to move the flow cell closer to the laser beam to find a sweet spot where the plane of light scattered by the stream is not broadened so much that it leaks around the obscuration bar. Jim Jett James H. Jett, Ph. D., Laboratory Fellow Director, National Flow Cytometry Resource Mail Stop M-888 Los Alamos National Laboratory Los Alamos, NM 87545 USA Phone: 505/667-3843 FAX: 505/665-3024 email: jett@lanl.gov The National Flow Cytometry Resource is an NIH/NCRR Resource Center.
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