From: Gerhard Nebe-von-Caron (Gerhard.Nebe-von-Caron@unilever.com)
Date: Mon Mar 30 1998 - 01:57:44 EST
The more recent Zynaxis PKH dyes (sold by Sigma) as well as
the carboxyfluorescein succinimidyl ester (CFS) (J.Cell
Biol.101, 610; ;1985 and 103,2649;1986) are both good
tracker dyes. I have advertised the latter for a long time,
and use it with bacteria for that purpose. When using the
succinimidyl ester dyes the carboxy-dichloro-fluorescein
diacetate succinimidyl ester (CCFAS, #LA-572, Lamda
fluorescence technology, Graz) has an advantage of a lower
pKa (4.5) thus fluorescence is less pH dependent. The
advantage of CCFAS is that initially only 'vital' cells are
labeled and the dye binds intracellular, but the dependence
of esterase activity also enhances variation and cell
volume. The PKH dyes bind to all cell membranes and might
(have not compared it) therefore give smaller CV's. Also
that cell wall is a square and cell volume the cube function
of variations in cell diameter might be of advantage. I was
always interested in a double labeling experiment of those
two cell tracker systems to see how daughter cells share
membranes and cytoplasm. Perhaps one day...
All the best
Gerhard.Nebe-von-Caron@unilever.com
______________________________ Reply Separator _________________________________
Subject: Long Term Vital Cel Staining
Author: Jim.Houston@stjude.org at INTERNET
Date: 27/03/1998 00:18
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noname has been scanned and no virus was found
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Anyone know of a fluorescent marker/stain that would survive culturing
of cells for 1 to 2 days. I would like to track these cells through
various depletion procedures.
Jim Houston
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