Re: 4 colors on FACS Calibur / PECy7

From: John Altman (altman@microbio.emory.edu)
Date: Sun Mar 29 1998 - 15:17:47 EST


How efficient is the transfer from PE to Cy7? In other words, are the
compensations from FL3 into FL2 huge?

Thanks,

John

Karenrt wrote:
> 
> Caltag Laboratories has just released several monoclonal antibodies conjugated
> to PE-Cy7 developed for just this purpose. There is virtually no cross-laser
> compensation between the PE-Cy7 and APC, since the Cy7 dye is not excited by
> the red diode laser on the FACSCalibur. This dye is also brighter than PerCP
> and peak emission is at 755 nm, with excitation at 488 nm. It is currently
> available conjugated to human CD4, CD8, CD45, Streptavidin, and two isotype
> controls (mouse IgG1 and IgG2a); more antibodies are being developed for
> future release. The list price is $200/ 0.5ml (minimum 100 tests) for CD4,
> CD8, and CD45, and $175 for the Streptavidin. Please contact us at (800)
> 874-4007 or your local sales representative for more information.
> 
> Karen R. Tamul, MS, MT(ASCP)SI
> Mid-Atlantic Territory Manager
> Caltag Laboratories, Inc
> (301) 540-3712 (voice & fax)
> e-mail: karenrt@aol.com

-- 
***********************************************************************
John Altman                          email: altman@microbio.emory.edu
Department of Microbiology           Office: (404) 727-5981
   and Immunology                    Lab:    (404) 727-8535
Emory University                     FAX:    (404) 727-3659
Rollins Research Center 3119
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Atlanta, GA 30322





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