cytometry data review

From: David McFarland (David.McFarland@mcmail.vanderbilt.edu)
Date: Fri Mar 13 1998 - 16:48:08 EST


I was contacted by someone today that is reviewing a paper containing some flow
data.  She asked some very basic questions about how to interpret a 2 parameter
cytogram (dot plot).  I gave her some advice on how to interpret the plot and
what info it was lacking, in my humble opinion.  I was pretty busy today so I
just gave her a short list of other info that should be included about flow
cytometry data collection.  Instrumentation, filters, lasers, # events, etc. 
Since the work wasn't done in my lab, I couldn't give her the specifics
directly.  Is there a resource somewhere to help reviewers in situations such as
these so they know what to look for?

Julie Auger:  If you see this, I think it is something that could go into
"educational ideas" bin.

David McFarland
Howard Hughes Medical Institute
Flow Cytometry Facility
Vanderbilt University Medical Center  


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