From: Witherspoon, Sam (sw11527@glaxowellcome.com)
Date: Thu Mar 12 1998 - 21:55:12 EST
Greetings All, Since Howard M. replied and kept this thread alive, I figured I would add caution to a note from MAK about "Hematall". >Eric, >I use Hematall Diluent (H-60620D, Valtech Diagnostics, 806 5th Ave, >Brackenridge PA 15014, phone 412-224-2440). It is simple PBS, and very >inexpensive. I sterilize it thru a .2 micron filter before use . . . no >problems. >MAK. >Eric Van Buren wrote: The "Hematall" I got from Fisher Scientific was azide-free (endo? they didn't say it was low endo.) but had an antimicrobial in it. I did not use it for sterile "recovery" sorts, especially into large pools. I don't know for sure if this is the same stuff as described by Mark. It was good for most analytical runs but could cause problems in Ca++ flux experiments. Sorry I can't recommend a "pre-diluted" source for you; I use a PBS concentrate myself, but have been tempted to switch to a "bag" system such as described by Howard. > -----Original Message----- > From: aa9080@wayne.edu [SMTP:aa9080@wayne.edu] > Sent: Tuesday, March 03, 1998 3:44 PM > To: Cytometry Mailing List > Subject: sterile, endotoxin-free sheath for sorting? > > > Does anyone have any suggestions for a source of sterile, endotoxin-free > sheath fluid for sorting? Our available water supply is not > endotoxin-free, > so we cannot dilute a concentrated solution. > > How about a source for sheath fluid for the FACScan? > > Thank you for your assistance. > Eric > > /\/\/\_ Eric Van Buren, aa9080@wayne.edu > \ \ \ Karmanos Cancer Institute and Immunology & Microbiology > \_^_/ Wayne State University, Detroit, Michigan, USA >
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