From: Theresa L. Fecik (flowcytoqueen@juno.com)
Date: Tue Mar 03 1998 - 16:32:13 EST
Nancy, If I have a specimen with a low cell count, and the pathologist REALLY wants me to try to do flow cytometry analysis on it, then I will increase the amount of specimen that I use per tube (if I have enough specimen to do that). We use less antibody per tube than the manufacturer calls for, anyway, to save money--instead of 100 ul of blood/bone marrow and 20 ul of antibody per tube, we use 50 ul of blood/bone marrow and 10 ul of antibody per tube (well.....O.K.--I use 100ul of sample and 10 ul of antibody--but, it seems to work just fine). For bone marrow and blood specimens that have a WBC count of 3000 or less, 100 ul would be double the amount of specimen for the amount of antibody being used, and I wouldn't have to put the tubes through a second lysing cycle. I personally don't want to play with the amount of antibody any more than it has already been played with. Hope this helps. Going with the flow, Terry Fecik Sinai Hospital of Baltimore _____________________________________________________________________ You don't need to buy Internet access to use free Internet e-mail. Get completely free e-mail from Juno at http://www.juno.com Or call Juno at (800) 654-JUNO [654-5866]
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