From: PETER BERCZ (BERCZ.PETER@epamail.epa.gov)
Date: Wed Feb 25 1998 - 07:53:33 EST
** Reply Requested When Convenient ** Sorry folks, in my previous posting I attached a WPWIN file about this topic, and it was apparently garbled up by the internet transmittal process. So here it is retyped: Several Estrogen Receptors (ER) probes were found in the open literature and their potential in studying endocrine disruptors were evaluated. a. 17-beta estradiol-6 carboxymethyloxime - bovine serum albumin -FITC conjugate For tagging membrane binding sites Pappas TC, Gametchu B, Watson CS, 1995 Membrane estrogen receptors identified by multiple antibody labeling and impeded ligand binding. FASEB J 1995 March. 9(5) 404-10 and Brubaker KD, Gay CV 1994, Specific binding of estrogen to osteoclast surfaces. Erratum in Biochem Biophys Res.Comm 1994 Jul 15; 202(1) 643, Main paper in ibid 1994 apr 29; 300(2):899-907 b. 7-alpha-pentyl, 17-betaestradiol-BODIPY conjugate a probe of high quantum yield, potential for flow cytometry French AN, Wilson SR, Welch MJ, Katzenellenbogen JA; 1993 Synthesis of 7-alpha-substituted estradiols: synthesis and biological evaluation of 7-alpha-pentyl substituted BODIPY fluorescent conjugate and a fluorine-18-labeled 7-alpha-pentyl estradiol analog. Steroids 1993 Apr ; 58(4) 157-69 c. 7',6-dihydroxycoumarino (3',4',3,2) coumarone or COUMESTROL Miksicek RJ; 1993, In situ localization of the estrogen receptor in living cells with the phytoestogen COUMESTROL J.Histochem-Cytochem 1993 Jun 41(6): 801-10 d. Tetrahydrochrysene complex with rigidified hydroxynitrostilbene fluorophore for cytosolic ER compartment mapping. Anstead GM, Hwang KJ, Katzenellenbogen JA 1993; Characterization of the spectroscopic properties of a tetrahydrocgrysene system containing a rigidified hydroxynitrostilbene chromophore; An inherently fluorescent ligand designed for the estrogen receptor, Photochem-Photobiol. 1993 April; 57(4):616-28
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