Re: FACS Convert

From: Janet E. Lewis (jelewis1@facstaff.wisc.edu)
Date: Tue Feb 10 1998 - 18:16:53 EST


Haywood,

>I saved the files I needed to analyze on a diskette on my HP, found the FACS 
>Convert folder in the BD Applications folder on the MAC and placed the      
>diskette in the MAC floppy port.  The MAC summarily rejected the diskette   
>and wanted to format it.

When the HP diskette was formatted, there are one of two ways to do it.
One is compatible with the PC (Windows) program HP Reader and one is
compatible with FACS Convert on the Mac.  Most likely, what you have are
diskettes formatted as 
format option 3, interleave factor 1 (which is the default our HP had).

If you don't have the networking set up (which, by the way, doesn't work
with the most current versions of MacTCP and OpenTalk), you can do what we
did.  Format some diskettes in the HP, but with format option 2, interleave
factor 2.  Copy your files from the old diskettes onto the HP hard drive.
Then copy those files onto your newly-formatted "Mac compatible" diskettes.  

If FACS Convert doesn't read those as are, then use HP-to-Mac, which you
can download from SoftFlow (as a free utility) at
http://www.wavefront.com/~soft-flow/.  It's also available at the
UMass/Amherst Flow Cytometry Facility web site's "Catalog of Free Flow
Cytometry Software" page at http://www.bio.umass.edu/mcbfacs/flowcat.html.  

After you've HP-to-Mac'ed the files into a folder on your Mac, then use
FACS Convert on them.  This method will definitely work, even if FACS
Convert doesn't like your disks.

The other option if you have a PC with HPReader (or an equivalent) is to
use HP Reader to copy the files to the PC.  If the PC and Mac are both on a
network, then you can transfer them (by FTP, for example) to the Mac.  If
one or both are not networked, then copy the files onto PC-formatted
floppies, and then copy those files from the PC floppies to your Mac hard
drive.  FACS Convert should work on the files on the Mac hard drive. 

Good luck! 

--Janet





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Janet E. Lewis
UWCCC Flow Cytometry Facility
Clinical Sciences Center, K4/535
600 Highland Ave.
Madison, WI  53792-0001

(608)263-0313
jelewis1@facstaff.wisc.edu
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