H33342 and RNA

From: DEEKEWB@aol.com
Date: Tue Feb 10 1998 - 13:05:50 EST

• Next message: Antony Bakke: "List reply-to setting. -Reply"
• Previous message: Steve Kelley: "Re: List reply-to setting."
• Next in thread: Howard Shapiro: "Re: H33342 and RNA"
• Maybe reply: Howard Shapiro: "Re: H33342 and RNA"
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]

Fellow flowers:

  Our goal is to use pyronin (PY) to stain live cells for RNA content.
Preliminary studies in our lab have suggested that quiescent hematopoietic
cells survive PY staining provided H33342 is not used simultaneously.  Thus,
we have been testing the importance of H33342 addition for specificity of PY
staining of RNA.  

This has led to 2 questions:
  1.  Does anyone have a good protocol to permeablize cells for RNase
treatment to quantitate the specificity of pyronin staining?
  2.  Can anyone explain the following? Cells were stained with PY, PKH67 and
PE-CY5 with or without H33342.  We found that addition of H33342 increased the
mean channel fluorescence of PY, PKH67 and PE-CY5 by 1.32-fold, 1.34-fold, and
1.44-fold respectively. How is this possible?  Any insight would be
appreciated.  Thanks a bunch.  
Perplexed in Portland.
Doug Dooley, 
ARC, 
Stem Cell Lab
Portland, OR
deekewb@aol.com


  

• Next message: Antony Bakke: "List reply-to setting. -Reply"
• Previous message: Steve Kelley: "Re: List reply-to setting."
• Next in thread: Howard Shapiro: "Re: H33342 and RNA"
• Maybe reply: Howard Shapiro: "Re: H33342 and RNA"
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:35:13 EST