From: John Altman (altman@microbio.emory.edu)
Date: Thu Feb 05 1998 - 13:13:07 EST
We've been having an odd problem with using PI to exclude dead cells on our FACS Calibur. When I used to use an old FACS Scan, the dead cells were very, very bright in PI, allowing me to use antibody reagents in FL2 and FL3 in addition to PI. On our Caliber, however, we observe a continuous distribution of PI+ cells (fresh splenocytes), starting with the PI-negatives all the way up to the bright cells. This makes it impossible, of course, to use other reagents in FL2 and FL3. We're using PI at 1 ug/ml final. Peter Katsikis at Allegheny also tells me that he's seen this on his Caliber, where he never saw it before. Any clues? Thanks, John -- *********************************************************************** John Altman email: altman@microbio.emory.edu Department of Microbiology Office: (404) 727-5981 and Immunology Lab: (404) 727-8535 Emory University FAX: (404) 727-3659 Rollins Research Center 3119 1510 Clifton Road Atlanta, GA 30322
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