Dendritic cells

From: J.Lawry@sheffield.ac.uk
Date: Tue Jan 27 1998 - 04:35:09 EST

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Dear All,

We are trying to characterise dendritic cells in stimulated cultures of
PBMCs. We have a panel of potential markers of dendritic cells but are
having problems finding their characteristic scatter patterns for gating
purposes. Back gating gives no clear area. Can anyone help? Where do these
cells lie on a normal FWD vs SSC plot of "whole blood" - in the
LGL/monocyte area, or towards the neutrophils?

Many thanks, John.

 Dr John Lawry,
Institute for Cancer Studies, University Medical School, Beech Hill Road,
Sheffield. S10 2RX.
Tel:0114-271-3023 (lab), 0114-271-3774 (office)
Fax:0114-271-3515
Email: J.Lawry@sheffield.ac.uk
Web page: http://www.shef.ac.uk/uni/academic/A-C/csi/Staff/John/JL.html

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