Re: Mithramycin protocl on Epics XL

From: Howard Shapiro (hms@shapirolab.com)
Date: Sat Jan 10 1998 - 21:41:32 EST

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Gerard Engelen-Eigles asks whether anyone has run Mithramycin stained cells
on an Epics XL.

Probably not successfully; mithramycin absorbs very poorly at 488 nm, which
is the only excitation wavelength available on the XL.  In instruments with
larger argon lasers, 457 nm is generally used; 441 nm from He-Cd lasers and
436 nm from arc lamps work as well or better.

-Howard

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