From: Gerhard Nebe-von-Caron (Gerhard.Nebe-von-Caron@unilever.com)
Date: Wed Dec 24 1997 - 04:37:55 EST
This article might be of your interest
Have a peaceful Christmas
Gerhard
SIMULTANEOUS 3-COLOR ANALYSIS OF THE SURFACE PHENOTYPE AND DNA-RNA
QUANTITATION USING 7-AMINO-ACTINOMYCIN-D AND PYRONIN-Y
TOBA K, WINTON EF, KOIKE T, SHIBATA A
JOURNAL OF IMMUNOLOGICAL METHODS 182: (2) 193-207 JUN 9 1995
______________________________ Reply Separator _________________________________
Subject: Pyronin/hoechst
Author: DEEKEWB@AOL.COM at INTERNET
Date: 19/12/97 14:49
Cell cyclers: two questions--
1. When viable cells are labeled for RNA content with pyronin Y and sorted
and returned to culture, how long does the stain remain associated with the
cells? I ask because of some concern that pyronin fluorescence might
interfere with subsequent antibody staining and analysis.
2. Is it possible to read pyronin Y staining after fixing with 0.5-1.0%
paraformaldehyde?
Thanks for the help.
Doug Dooley, American Red Cross, Portland, Oregon
deekewb@AOL.com
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