Pyronin/hoechst

From: DEEKEWB (DEEKEWB@aol.com)
Date: Thu Dec 18 1997 - 23:52:40 EST

Next message: medbury@renal.wsahs.nsw.gov.au: "Apoptosis again....."
Previous message: Gerhard Nebe-von-Caron: "Re: Nile Red and Aquatic Organisms"
Next in thread: Howard Shapiro: "Re: Pyronin/hoechst"
Maybe reply: Howard Shapiro: "Re: Pyronin/hoechst"
Maybe reply: Gerhard Nebe-von-Caron: "Re: Pyronin/hoechst"
Maybe reply: Gerhard Nebe-von-Caron: "Re: Pyronin/hoechst"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]

Cell cyclers: two questions--
  1. When viable cells are labeled for RNA content with pyronin Y  and  sorted
and returned to culture, how long does the stain remain associated with the
cells?  I ask because of some concern that pyronin fluorescence might
interfere with subsequent antibody staining and analysis.
  2.  Is it possible to read pyronin Y staining after fixing with 0.5-1.0%
paraformaldehyde?
  Thanks for the help. 
  Doug Dooley, American Red Cross, Portland, Oregon
  deekewb@AOL.com

Next message: medbury@renal.wsahs.nsw.gov.au: "Apoptosis again....."
Previous message: Gerhard Nebe-von-Caron: "Re: Nile Red and Aquatic Organisms"
Next in thread: Howard Shapiro: "Re: Pyronin/hoechst"
Maybe reply: Howard Shapiro: "Re: Pyronin/hoechst"
Maybe reply: Gerhard Nebe-von-Caron: "Re: Pyronin/hoechst"
Maybe reply: Gerhard Nebe-von-Caron: "Re: Pyronin/hoechst"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:34:08 EST