From: David Lloyd (lloyddr@novell2.bham.ac.uk)
Date: Mon Nov 17 1997 - 12:34:55 EST
Dear all Thank you to all who replied to my questions, especially those who were able to supply references. For your information: The majority of respondents said that ethanol fixed cells should be fine for cell cycle analysis if held at ambient temperature in transit; one said that using ice would be better and one suggested an alternative fixative. The answers from respondents to the question of DNA content of normal lymphocytes were more variable; from 3 to 8 pg/cell; presumably reflecting different methods of measuring DNA content? Alternatives proposed by some respondents were very interesting and we are now investigating those approaches. Also; Tom Frey please note; one of eight DNA content restpondents did reply in "modern units", giving a value of 3.3 e+9 base pairs per cell. Thanks again for all your help David R Lloyd University of Birmingham England t; +44 (0)121 414 5264/7 e; D.R.Lloyd@bham.ac.uk
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