From: Mayumi Naramura (MNARAMURA@atlas.niaid.nih.gov)
Date: Sun Nov 02 1997 - 13:20:33 EST
I'm trying to study apoptosis of 1. fresh mouse thymocytes induced by 2. TCR stimulation using 3. flow cytometry, either by 3a. Annexin V or by 3b. TUNEL. I know there are tremendous amount of literature related to this, but so far I have not come across with ones that contain all the "keywords" I listed above. Specifically, I found most of the study has been done using "immature" T cell lines or hybridomas instead of "fresh mouse thymocytes". I tried it a couple of times, but I cannot see any difference between cells stimulated with anti-CD3 and non stimulated control because a significant portion of cells are Annexin V (+) in the control. Any suggestions? Mayumi Naramura, M.D. NIH/NIAID Lab of Immunology Twinbrook II, Rm. 125 12441 Parklawn Drive Rockville, MD 20852 Phone: 301-402-4595 FAX: 301-594-2522 e-mail:mnaramura@atlas.niaid.nih.gov
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