Apoptosis vs oncosis

From: LAADAMS@am.pnu.com
Date: Thu Oct 30 1997 - 11:55:19 EST

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Hello group!

This is a basic science question.  Does anyone have a definition of oncosis 
which differentiates this method of cell death from apoptosis, including 
morphological and/or biochemical hallmarks?  I have been treating HT29 
(human colon adenocarcinoma) cells with a potential antitumor drug and I 
don't think this agent is killing the cells by apoptosis.  Others in my 
group do not agree.  The cells start to swell after 24-48 hours of 
treatment and by 96 hours the most of the culture is dead.  At no point in 
the time course do the cells demonstrate true positive TUNEL results.  I 
have only looked at DNA laddering once and got ambiguous results, i.e., I 
think I looked at too late of a time point.

Any and all comments/suggestions would be appreciated before I repeat any 
more experiments.  Thanks!

Lisa Adams
Pharmacia & Upjohn
Kalamazoo, MI
LAADAMS@am.pnu.com

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