From: gaston@MIT.EDU
Date: Sun Jul 13 1997 - 18:09:29 EST
Hi I may need to post this question to a different list, but I'm hoping that someone in flow-nation can point me in the right direction. One of the fellows in the lab is trying to get transfected expression of HLA-A3 (pHeBo vector, neo selection) in lymphoblastoid cells, and has been having problems that seem to be the result of re-arrangements in the plasmid. It appears that these re-arrengements occurred before this fellow received it. Does anyone know where we might obtain another HLA-A3 expression vector, ideally with neo selection (but any reliable selection agent would be usable)? If so, could you send me the name/address/email etc of the appropriate contact person? Thank you for your help! Sandra M. Gaston PhD Center for Cancer Research MIT gaston@mit.edu
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