Identification of Eosinophils in Flow Cytometry -Reply

From: ANDRZEJ DEPTALA (ANDRZEJ_DEPTALA@nymc.edu)
Date: Tue Jun 17 1997 - 19:08:10 EST

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You can use simultaneous detection: FLS/SS and CD45/CD14 MoAb
staining. The CD45-FITC/CD14PE bivariate distribution shows CD45-dull
cells, CD45-dim cells, CD45-bright cells and CD14+ cells. The FLS
versus SS  distribution of CD45-bright cells shows eosinophils, which
are the most CD45-bright and CD14- among the granular cells. 
Andrzej Deptala
The Cancer Research Institute, NYMC, Valhalla, USA

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