post sorting fibroblast viability

From: @rmslab.rockefeller.edu
Date: Thu Feb 06 1997 - 17:47:22 EST

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dear colleagues

does anyone have experience sorting fibroblast cell lines?

i'm sorting FLST cell line. this is an embryonic cell line which supports b-cell development.
unfortunately after sorting the cells remain alive but don't attach like they
normally do

i detach the cells by cell scraping and filter through a mesh post two step staining.

cytometry is on a facsvantage, 70 micron nozzle, sheath p.s.i is 11.

any ideas for increasing viablity/adherence would be greatly appreciated.


frank isdell
flow cytometry lab.
the rockefeller university
1230 york ave
ny

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