FAS and FAS-LIGAND ANTIBODIES as of: 19. Dec 1996 FAS-Antigen (CD95, APO-1) Fas-Ligand (CD95L, APO-1L) Product Name Clone Isotyp Host Target Presentation Amount Features Epitope application Order No. Manufacturer net USD net DEM Price DEM Personal Comments Product Name Clone Isotyp Host Target Species Presentation Amount Features Epitope Applications Order No. Manufacturer Provider net Price USD net Price DEM Price DEM Personal Comments anti-FAS UB2 IgG1 mouse H liquiid purified 100 µg none surface FCM, frozen section Immunotech 658,00 DM 756,70 DM no information available Human Fas-Ligand NOK-1 IgG1 mouse H liquid purified 100 µg neutralizing surface (membrane+soluble FasL) IP, FCM, ELISA 65321A Pharmingen 450,00 DM 517,50 DM "NOK-1 gives a bright staining on T-cell clones and few B-cell leukemias on flow cytometry; functional assays not tested personally" anti-FAS UB2 IgG1 mouse H FITC conjugated 50 Tests (1 mL) none surface FCM, frozen section 1506 Immunotech 658,00 DM 756,70 DM functions perfectly on flow cytometry when used for expression determination (own experience) Human Fas-Ligand NOK-1 IgG1 mouse H biotinylated 100 Tests (1 mL) neutralizing surface (membrane+soluble FasL) IP, FCM, ELISA 65322X Pharmingen 540,00 DM 621,00 DM "NOK-1 gives a bright staining on T-cell clones and few B-cell leukemias on flow cytometry; functional assays not tested personally" anti-FAS UB2 IgG1 mouse H PE conjugated 50 Tests (1 mL) none surface FCM, frozen section 1739 Immunotech 688,00 DM 791,20 DM functions perfectly on flow cytometry when used for expression determination (own experience) Human Fas-Ligand NOK-1 IgG1 mouse H no azide/low endotoxin 250 µg neutralizing surface (membrane+soluble FasL) IP, FCM, ELISA 65320C Pharmingen 484,00 DM 556,60 DM "NOK-1 gives a bright staining on T-cell clones and few B-cell leukemias on flow cytometry; functional assays not tested personally" anti-FAS ZB4 IgG1 mouse H liquiid purified 100 µg inhibits apopto surface FCM, culture, frozen 1737 Immunotech 658,00 DM 756,70 DM "personal experience shows contradicting results; some apoptosis induction is inhibited, some not." FAS-Ligand (CD95L) 33 IgG1 mouse H lyophilized 150 µg cytoplasm (C-terminal aa 116-277) WB, IF, (FCM) F37720 Transduction Laboratories Dianova 33 gives bright and clear staining when cells are sufficiently permeabilized and fixed prior to flow cytometric staining anti-FAS CH11 IgM mouse H liquiid purified 50 µg induces apoptos surface FCM, WB, cell culture 1504 Immunotech 658,00 DM 756,70 DM personal experience shows that CH11 functions perfectly as inducer of apoptosis FAS-Ligand (CD95L) 33 IgG1 mouse H lyophilized 350 µg cytoplasm (C-terminal aa 116-277) WB, IF, (FCM) F37720 Transduction Laboratories Dianova 33 gives bright and clear staining when cells are sufficiently permeabilized and fixed prior to flow cytometric staining CD95 (FAS/APO-1)DX2 IgG1 k mouse H liquiid purified 100 µg induces apoptos surface FCM, frozen section 33451A Pharmingen 390,00 DM 448,50 DM no information available FAS-Ligand (Ab-2) ??? (Ab-2) IgG1 kappa mouse M (H) lyophilized 100 µg cytoplasm (C-terminal aa 261-277) WB AM-12 Oncogene/Calbiochem Dianova "to my knowledge not available in Germany yet; see further remarks (1)" CD95 (FAS/APO-1)DX2 IgG1 k mouse H FITC conjugated 100 Tests (1 mL)induces apoptos surface FCM, frozen section 33454X Pharmingen 500,00 DM 575,00 DM colleague's experience on T-cells shows a very good staining in flow cytometry Human Fas-Ligand NOK-2 IgG2a mouse H no azide/low endotoxin 250 µg neutralizing surface (membrane+soluble FasL) IP, FCM, ELISA 65330C Pharmingen 450,00 DM 517,50 DM not tested personally CD95 (FAS/APO-1)DX2 IgG1 k mouse H PE conjugated 100 Tests (1 mL)induces apoptos surface FCM, frozen section 33455X Pharmingen 550,00 DM 632,50 DM no information available Human Fas-Ligand NOK-2 IgG2a mouse H liquid purified 100 µg neutralizing surface (membrane+soluble FasL) IP, FCM, ELISA 65331A Pharmingen 848,00 DM 975,20 DM not tested personally CD95 (FAS/APO-1)DX2 IgG1 k mouse H biotinylated 100 Tests (1 mL)induces apoptos surface FCM, frozen section 33452X Pharmingen 650,00 DM 747,50 DM no information available Human Fas-Ligand G247-4 IgG1 mouse H liquid purified 100 µg surface (membrane+soluble FasL) WB, IP, ELISA, FCM 65431A Pharmingen 450,00 DM 517,50 DM not tested personally CD95 (FAS/APO-1)DX2 IgG1 k mouse H no azide/low endotoxin 500 µg induces apoptos surface FCM, frozen section 33540D Pharmingen 848,00 DM 975,20 DM no information available FAS-L (N-20) polyclonal IgG rabbit M, R, H liquid purified 100 µg surface (N-terminal aa 2-21) WB, IC sc-834 Santa Cruz Biotechnologies no information available FAS CD95 7C11 IgM mouse H RD1 conjugated 50 Tests (1 mL) none surface FCM Immunotech - DM no information available FAS-L (Q-20) polyclonal IgG rabbit H, R, M liquid purified 100 µg surface (N-terminal aa 2-21) WB, IC sc-956 Santa Cruz Biotechnologies no information available anti-FAS UB2 IgG1 mouse H liquiid purified 50 Tests (1 mL) none surface FCM, frozen section Kamiya 280,00 - DM "no personal experience available; if it is functioning like Immunotech's version, then same comment applies" FAS-L (C-20) polyclonal IgG rabbit H liquid purified 100 µg cytoplasm (C-terminal aa 260-279) WP, IC sc-957 Santa Cruz Biotechnologies no information available anti-FAS UB2 IgG1 mouse H FITC conjugated 50 Tests (1 mL) none surface FCM, frozen section Kamiya 290,00 - DM "no personal experience available; if it is functioning like Immunotech's version, then same comment applies" anti-FAS UB2 IgG1 mouse H PE conjugated 50 Tests (1 mL) none surface FCM, frozen section Kamiya 260,00 - DM "no personal experience available; if it is functioning like Immunotech's version, then same comment applies" anti-FAS ZB4 IgG1 mouse H liquiid purified 100 µg inhibits apopto surface FCM, culture, frozen Kamiya 260,00 - DM "no personal experience available; if it is functioning like Immunotech's version, then same comment applies" anti-FAS CH11 IgM mouse H liquiid purified 100 µg induces apoptos surface FCM, WB, cell culture Kamiya 260,00 - DM no information available anti-FAS SM1/1 IgG2a mouse H liquiid purified 50 µg induces apoptos surface FCM, IF Kamiya 260,00 - DM no information available anti-FAS SM1/17 IgG2b mouse H liquiid purified 100 Tests (1 mL)none surface FCM Kamiya - DM no information available anti-FAS SM1/23 IgG2b mouse H liquiid purified 50 µg inhibits apopto surface FCM Kamiya 195,00 - DM no information available anti-FAS SM1/1 IgG2a mouse H liquiid purified 50 µg induces apoptos surface FCM, IF Bender MedSystems - DM no information available anti-FAS SM1/17 IgG2b mouse H liquiid purified 100 Tests (1 mL)none surface FCM Bender MedSystems - DM no information available anti-FAS SM1/23 IgG2b mouse H liquiid purified 50 µg inhibits apopto surface FCM Bender MedSystems - DM no information available human Fas G254-274IgG1 mouse H liquiid purified 100 µg none surface ELISA capture mAb, WB 65311A Pharmingen - DM no information available CD95 (human FAS)B-G27 IgG2a mouse H liquiid purified 200 Tests (2 mL)induces apoptos surface FCM CBL527 Cymbus Bioscience 358,00 DM 411,70 DM no information available CD95 (human FAS)B-G27 IgG2a mouse H FITC conjugated 200 Tests (2 mL)induces apoptos surface FCM CBL527F Cymbus Bioscience 408,00 DM 469,20 DM no information available CD95 (human FAS)B-G27 IgG2a mouse H biotinylated 100 µg induces apoptos surface FCM CBL527B Cymbus Bioscience 408,00 DM 469,20 DM no information available CD95 (human FAS)B-G27 IgG2a mouse H no azide/low endotoxin 500 µg induces apoptos surface FCM, cell culture CBL527N Cymbus Bioscience 844,00 DM 970,60 DM no information available Fas (Ab-2) DX2 IgG1 k mouse H lyophilized 100 µg induces apoptos surface FCM, IF AM01 Calbiochem/Oncogene 478,00 DM 549,70 DM no information available Fas (Ab-3) DX3 IgG2a k mouse H lyophilized 100 µg unknown surface FCM, IF AM02 Calbiochem/Oncogene 478,00 DM 549,70 DM no information available Fas-Ligand (CD95L, APO-1L) Product Name Clone Isotyp Host Target Species Presentation Amount Features Epitope Applications OrderNo.Manufacturer net USD net DEM DEM Personal Comments Human Fas-Lig NOK-1 IgG1 mouse H liquid purified 100 µg neutralizing surface (membrane+soluble FasL) IP, FCM, ELISA 65321A Pharmingen 450,00 DM 517,50 DM NOK-1 gives a bright staining on T-cell clones and few B-cell leukemias on flow cytometry; functional assays not tested personally Human Fas-Lig NOK-1 IgG1 mouse H biotinylated 100 Tes neutralizing surface (membrane+soluble FasL) IP, FCM, ELISA 65322X Pharmingen 540,00 DM 621,00 DM NOK-1 gives a bright staining on T-cell clones and few B-cell leukemias on flow cytometry; functional assays not tested personally Human Fas-Lig NOK-1 IgG1 mouse H no azide/low endotoxin 250 µg neutralizing surface (membrane+soluble FasL) IP, FCM, ELISA 65320C Pharmingen 484,00 DM 556,60 DM NOK-1 gives a bright staining on T-cell clones and few B-cell leukemias on flow cytometry; functional assays not tested personally FAS-Ligand 33 IgG1 mouse H lyophilized 150 µg cytoplasm (C-term aa 116-277) WB, IF, (FCM) F37720 Transduction Laboratori 33 gives bright and clear staining when cells are sufficiently permeabilized and fixed prior to flow cytometric staining FAS-Ligand 33 IgG1 mouse H lyophilized 350 µg cytoplasm (C-term aa 116-277) WB, IF, (FCM) F37720 Transduction Laboratori 33 gives bright and clear staining when cells are sufficiently permeabilized and fixed prior to flow cytometric staining FAS-Ligand ?(Ab-2) IgG1 k mouse M (H) lyophilized 100 µg cytoplasm (C-terml aa 261-277) WB AM-12 Oncogene/Calbiochem to my knowledge not available in Germany yet; see further remarks (1) Human Fas-Lig NOK-2 IgG2a mouse H no azide/low endotoxin 250 µg neutralizing surface (membrane+soluble FasL) IP, FCM, ELISA 65330C Pharmingen 450,00 DM 517,50 DM not tested personally Human Fas-Lig NOK-2 IgG2a mouse H liquid purified 100 µg neutralizing surface (membrane+soluble FasL) IP, FCM, ELISA 65331A Pharmingen 848,00 DM 975,20 DM not tested personally Human Fas-Lig G247-4 IgG1 mouse H liquid purified 100 µg surface (membrane+soluble FasL) WB,IP,ELISA,FCM 65431A Pharmingen 450,00 DM 517,50 DM not tested personally FAS-L (N-20) polycl IgG rabbit M, R, H liquid purified 100 µg surface (N-terminal aa 2-21) WB, IC sc-834 Santa Cruz Biotechnolog no information available FAS-L (Q-20) polycl IgG rabbit H, R, M liquid purified 100 µg surface (N-terminal aa 2-21) WB, IC sc-956 Santa Cruz Biotechnolog no information available FAS-L (C-20) polycl IgG rabbit H liquid purified 100 µg cytoplasm (C-terminal aa 260-279) WP, IC sc-957 Santa Cruz Biotechnolog no information available Abbreviations: Applications "FCM: flow cytometry; IF: Immunofluorescence; ICH: Immunohistochemistry; WB: Western Blotting; IP: Immunoprecipitation" Target Species "H: Human; M: Mouse; R: Rat; MO: Monkey (Simian); RB: Rabbit" Remarks: (1) According to information I recieved by product manager of the German distributor (Dianova) of Calbiochem/Oncogene apoptosis product series, this monoclonal antibody against the fas-ligand is specific for the murine protein and N O T (!!!) for human. It is raised against the murine amino acid residues 261-277 that are to be found within the cytoplasmic domain. Interestingly, the catalogue indicates human reactivity on an other page. GENERAL REMARKS:Prices are indicated only if information about US dollar pricing was available to me. Some companies provided me a price list in DEUTSCHMARKS, but as my anticipated most (flow) cytometrists do not reside in Germany. Therefore anyone interested in these antibodies have to inquire for prices not indicated in aforementioned listings. All information is either acquired by phone calls to companies and/or slaes representatives or is drawn from catalogues available to me. No liability or responsebility can be taken for information provided here, though I tried to be as precise as I could. Because of growing interest in apoptosis, any further information or update on these listings are hereby cordially welcomed. Commercial providers (manufacturers/distributors) mentioned above Kamiya Biomedical 910 Industry Drive, Tukwila, WA 98188, USA FON: +1-206-575-8068 FAX: +1-206-575-8094 WEB: NONE Immunotech US residents: inquire Coulter Corp., Hileah, FL, USA WEB: http://www.immunotech.com/ Transduction Laborat 2134 Nicholasville Rd., Suite 18, Lexington, KY 40503, USA FON: +1-606-278-1399 +1-800-227-4063 (both orders) FON: +1-606-278-7836 (technical assistance) FAX: +1-800-276-2251 (orders) WEB: http://translab.com/ Oncogene 80 Rogers St., Cambridge, MA 02142, USA FON: +1-617-492-7289 or +1-800-662-2616 FAX: +1-617-492-8438 or +1-800-828-4871 WEB: http://www.oncogene.com/ E-Mail: resprod@oncogene.com Calbiochem 84 Rogers St., Cambridge, MA 02142, USA FON: +1-617-577-9333 or +1-619-450-9600 or +1-800-662-2616 or +1-800-662-2616 FAX: +1-800-828-4781 or +1-617-577-8015 WEB: http://www.calbiochem.com/ PharMingen 10975 Torreyana Rd, San Diego, CA 92121, USA FON: +1-619-677-7737 or +1-800-848-6227 (customer service) FAX: +1-619-677-7749 or +1-800-825-5832 (technical service) WEB: http://www.pharmingen.com/ Bender MedSystems Dr. Boehringer Gasse 5-11, A-1121 Vienna, AUT FON: +43-1-80105-378 FAX: +43-1-80105-477 Cymbus Biosciences Unit J, Eagle Close, Chandlers Ford, Hants, S053 4NF, UK FON: +44-1703-267676 FAX: +44-1703-267677 Santa Cruz Biotechn 2161 Delaware Ave., Santa Cruz, CA 95060, USA FON: +1-408-457-3800 or +1-800-457-3801 FAX: +1-408-457-3801 WEB: http://www.scbt.com/ E-Mail: scbt@netcom.com Andreas Christaras Heinrich-Heine University Children's Hospital Duesseldorf Lab. of Experimental Hematology (KMT-Labor) Gebaeude (Bldg.) 14.82 Moorenstrasse 5 40225 Duesseldorf - GERMANY FON : +49-211-81-16103 FAX : +49-211-81-16191 E-Mail : christar@uni-duesseldorf.de