Beyond Packed Attributes1(shcherbina Message EncodingISO-8859Use Proportional FontsTextvNeed help with staining of human neutrophils for fluorescent microscopy (cytoskeletal proteins).My protocol works fine for platelets and lymphocytes, but not for neutrophils. After fixing with 2% paraformaldehyde and permeabilizing with 0.2-05 % Triton cells are negative even for actin. I tried another protocol: fixing with 1% glutaraldehyde, permeb. with 0.1% NP40 - stains fine for actin, but negative control is as bright as specific staining. My E-mail adress:shcherbina@cbr.medharvard.edu Anya Shcherbinav!V8MS Sans Serif8"MS Sans SerifwdH4 |  h T Attachment Count