From: Kees Pot (kpot@bccancer.bc.ca)
Date: Tue Dec 31 1996 - 13:52:00 EST
---------- From: Randy Gascoyne To: cyto-inbox Subject: RE: hairy cell leukemia Date: Tuesday, December 31, 1996 10:26AM Dear Dr Mann: This is my 1st time answering a question on the net. I usually just read the chat. Over the years I have used CD103 and find that it is expressed in all kinds of chronic B-cell leukemias, particularly the CD11c only variety ( ie SLVL, marginal zone LPD, leukemic Maltomas etc).. I would not make a diagnosis of HCL with TRAP negativity and absent CD25 and 11c. I have seen several hundred cases of HCL over the last 8-10 years, but none without CD11c and CD25. Most also express CD103. However, CD103 is certainly not specific for HCL. I have found CD103 expressed in a number of so-called HCL look- alikes. What about monocytopenia and/ or BM histology. What do the cells in the PB look like? HCL has a characteristic nuclear morphology. The more difficult problem are the cases of CD11c only disease that lack CD25, usually TRAP negative, but may express CD103. Are these HCL-variants or something else? Randy Gascoyne Hematopathologist ---------- »From: Kees Pot »To: Randy Gascoyne »Subject: FW: hairy cell leukemia »Date: Tuesday, 31 December, 1996 9:21AM » » » ---------- »From: mann0002@mc.duke.edu »To: Cytometry Mailing List »Subject: hairy cell leukemia »Date: Tuesday, December 31, 1996 6:36AM » » »I received peripheral blood for immunophenotyping for a question of hairy cell »leukemia. (The patient is an elderly male with splenomegaly and circulating »"hairy cells".) There is bright expression of the pan B cell markers CD19, CD20 »and CD22 as well as lambda light chain. The cells are negative for CD5 and CD10. »Although there is expression of CD103, the cells are negative for CD11c and »CD25. The cells are reportedly TRAP negative. » »I know that some people consider staining for CD103 highly specific for hairy »cells, but the absence of CD11c is very worrisome to me. What other B cell »neoplasms have you found to be CD103 positive? Would anyone consider a »diagnosis of hairy cell leukemia in the absence of staining for CD11c. » »Karen Mann »
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