kappa/lambda/CD19 reagents

From: Eric Hsi (ehsi@luc.edu)
Date: Tue Dec 31 1996 - 13:05:57 EST

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I have recently discovered this site. I have a question regarding 
reagents.  We have been testing the Caltag monoclonal kappa/lambda/cd19 
antbody cocktail and comparing it to the same BD combination to try and 
get a brighter CD19 signal for use in a clinical flow lab.  I have 
noticed a couple of cases in which the BD reagent shows no evidence of a 
light chain restriction but the Caltag reagent does (usually a very low 
percentage, dim kappa).  Has anyone else seen this?  The population 
could not be confirmed with polyclonal kappa and lambda from BD or Tago.

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