annexin V and RBC's

From: KATHY@flowcyt.cyto.purdue.edu
Date: Thu Dec 19 1996 - 15:19:25 EST

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Greetings fellow cytometrists,

Has anyone out there in flowland used annexin V to look at 
apoptosis in red blood cells? I have a researcher who is looking at 
apoptosis in canine RBC's. She is using a commercial source of 
annexin V (FITC labelled), and A23187 to induce the translocation of 
PS to the cell surface. We are getting no staining whatsoever. 
Any input would be greatly appreciated.

Thanks in advance!
Kathy Ragheb
 






Kathy Ragheb
Purdue University Cytometry Laboratories
HANS B050, West Lafayette, IN. 47907
ph: 317-494-0757   fax: 317-494-0517
ragheb@flowcyt.cyto.purdue.edu
http://www.cyto.purdue.edu

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