Re: PI staining of PBLs

From: Dr. Michael G. Ormerod (ormerod@icr.ac.uk)
Date: Thu Dec 19 1996 - 10:27:57 EST 

Thank you for the suggestion. We did use fresh blood. The 
difference observed was a lymph/gran difference (confirmed by cell 
sorting). We will try changing the coagulant.

Michael Ormerod

On Wed, 11 Dec 1996 11:07:41 -0800 "Dr. Philip McCoy" <pmccoy@UMDNJ.EDU> wrote:
> Dr. Michael G. Ormerod ;
> 
> What was your anticoagulant and how old was the blood? MOst likely you
> have a lymph/gran difference. Try using real fresh blood (<2hrs old) in
> Na-heparin.  Good luck!
> 
> Phil
> -- 
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> Flow Cytometry Laboratory, Three Cooper Plaza, Suite 410, Camden, NJ
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> J. Philip McCoy, Jr., Ph.D.                                           
> Director of Flow Cytometry                                            
> Associate Professor of
> Pediatrics                                          
> Tel: (609) 342-2227                                         
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