From: Dan Smith (DJS@ALLP.COM)
Date: Tue Dec 17 1996 - 12:25:00 EST
I have tried to use THP-1's in a cytochrome-C oxidative burst experiment and
had absolutely no luck. They may produce little to no superoxide.
Dan Smith
San Diego
----------
{From: owner-cyto-sendout
{To: Cytometry Mailing List
{Subject: THP-1's and fluorescent dyes
{Date: Monday, December 16, 1996 6:08PM
{
{
{
{ Has anyone worked with the THP-1 cell line using Dihydrorhodamine 123
{(DHR)
{ or Hydroethidine (HE)? I am in search of a method to stimulate these
{cells
{ with LPS, an inflammatory cytokine, or virus, and measure oxidative
{burst.
{ As of yet, my efforts have come up empty, but I thought there may be
{some
{ unpublished or hard to find methods out there in Flowland. Any help,
{
{ insights or watch-outs will be appreciated.
{
{ Thanks...Jodi McKenzie-Kroeger
{
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