From: Beavis, Andrew (abeavis@molecular.princeton.edu)
Date: Mon Dec 16 1996 - 12:10:00 EST
We have used several different fluorescent particles for detection of various cell surface antigens. The polystyrene particles were labelled with antibodies by passive adsorption, blocked with excess BSA and used in a variety of immunofluorescence assays. The SKY BLUE particles (0.4 - 2.0 um) from Spherotech have a unique emission at 730 nm and permitted six-colour immunofluorescence analysis (see Beavis and Pennline; BioTechniques, vol 21 No. 3, pp 498). The SKY BLUE particles are excited with either a 633 nm or 647 nm laser line and with the lower levels of autofluorescence in the red region, may provide advantages for detection of antigens expressed at low levels. The TRANSFLUOSPHERES 488/720 from Molecular Probes can be excited at 488 nm with emission at 720 nm. Although there is significant emission around 525 nm through 625 nm, these particles can also be used in combination with other 488-excited fluorochromes for multicolour analysis. The LIGHT YELLOW fluorescent particles from Spherotech can be excited with a UV laser ( 351-364 nm) with emission at around 400 nm. Despite high levels of autofluorescence, these labelled particles were also useful for cell surface antigen detection. Regards Andrew Beavis Manager, Flow Cytometry Core Facility Princeton University Department of Molecular Biology Lewis Thomas Laboratory Washington Road Princeton, NJ 08544 Tel. (609) 258 1695 e-mail. Abeavis@molecular.princeton.edu
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:31:33 EST
