Re[2]: apoptosis antibodies (FASL, GRANZ, PERFOR)

From: tom_frey@bdis.com
Date: Fri Dec 13 1996 - 16:53:31 EST

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Andreas Christaras
 
Thanks for providing the information below regarding your experience with anti-
fas-ligand mAbs.  However, I had the impression (possibly mistaken) that at 
least one of these mAbs recognized an intracellular epitope.  Could you clarify 
which are able to stain cells without premeabilization?

Tom_Frey@bdis.com
  
Dear Maria,

following companies provide antibodies against FAS-L (CD95L)

Transduction Laboratories       http://translab.com             clone 33
        in my experience on flow cytometry this mAb works fine
        available in purified unlabelled format

PharMingen                      http://www.pharmingen.com       clone NOK-1
        this is the antibody I regularly use and it works perfectly
        available in purified unlabelled format and biotinylated
                 
PharMingen                      http://www.pharmingen.com       clone NOK-2
        I have no experience using this antibody
        available in purified unlabelled format


Hope this helps a bit

Andreas Christaras
Heinrich-Heine University Children's Hospital
Laboratory of Experimental Hematology and Stem Cell Transplanatation
KMT-Labor Building 14.82
Moorenstrasse 5
40225 DUESSELDORF
Germany

FON     +49-211-81-16103        FAX     +49-211-81-16191        
E-M     christar@uni-duesseldorf.de

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