From: Wendy Jones (wjones@hawaii.edu)
Date: Fri Dec 13 1996 - 17:34:33 EST
Its nice to know that there are other plant flow-ers out there. John, There are three references you should probably take a look at if you haven't already: Dickson et al. 1992, Nuclear DNA content variation within the Rosaceae. American Journal of Botany. 79:1081-1086. They used the protocol found in: Arumuganathum and Earle. 1991. Estimation of nuclear DNA contents of plants by flow cytometry. Plant Molecular Biology Reporter. 9:229-233. This protocol uses PI (plus RNase). The third referece uses H0: Moyne et al. 1993. Relationship between cell ploidy and regeneration capacity of long term Rosa hybrida cultures. Plant Science. 93:159-168. Good luck! Wendy
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