From: Charles Kuszynski (CKUSZYNS@MAIL.UNMC.EDU)
Date: Fri Dec 13 1996 - 12:19:46 EST
I do not know much about the mollusk, but we have routinely examined blood cells from frogs. These animals can be induced to have 3N configurations. The protocol we use for staining is the procedure of Vindelov, LL 1977 Virchows Arch. B Cell. Path. 24:225-242 Basically, it is a 0.1% NP40 with RNAse (3.5 U/ml) and 0.75mg/ml PI in TRIS. Charles Kuszynski, Ph. D. Cell analysis Facility Univ. Nebr. Med. Cntr. Omaha, NE 68198-6495 (402) 559-6267
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