From: Iona O'Brien (iobrien@marccri.marc.cri.nz)
Date: Fri Dec 13 1996 - 14:34:29 EST
Hi, I have had experience with several plant species producing high levels of phenolics eg apple, kiwifruit and hops. I use either 0.1% ascorbic acid or 3% PVP-10 in the preparation buffer. The PVP-10 works best but can make the nuclei sticky. I have found better permeability with Hoescht 33342 than DAPI into apple fruit cells which were high in phenolics - so probably worth a try. I would also run an unstained sample of your cells as my past experience has been than when high levels of phenolics are present it can result in high levels of autofluorescence resulting in incorrect DNA content values. Hope this helps, Iona O'Brien
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